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. 2014 Aug 18;9(8):e105223. doi: 10.1371/journal.pone.0105223

Figure 4. Molecular analyses of L. incisa clones transformed with a pRbcS450 construct.

Figure 4

(A) PCR analysis: gDNA was amplified with BleF and BleR primers yielding a 415-bp fragment. Lanes: (M) DNA ladder; (-) no template, (Ble) ble plasmid control, (wt) negative control (non-transformed cells); five transformed clones. (B) Southern blot analysis: gDNA isolated from both transgenic and non-transgenic cells, as well as plasmid DNA (pRbcS450), were digested with KpnI restriction enzyme. The blot was hybridized with a probe derived from a 415-bp amplified fragment of the ble gene. Lanes: (M) 1 Kb ladder; (plasmid) positive control; five transformed clones; (wt) negative control (non-transformed cells).