Figure 2.
Dose dependent effect of soluble heparin (hep) on myogenic differentiation of C2C12 cells: MyHC immunofluorescent staining of C2C12 cultured with different concentrations of heparin in growth medium (top row) and differentiating medium (bottom row). Higher number of MyHC positive cells indicating differentiated cells, was observed in cultures containing higher heparin concentrations, while culture medium containing lower heparin concentration promoted multi-nucleated myotube formation. (Scale bar: 100μm)