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Dose dependent effect of PSS on myogenic differentiation of C2C12 cells: MyHC immunofluorescent staining of C2C12 cultured with different concentrations of PSS in growth medium (top row) and differentiating medium (bottom row). Similar to heparin containing culture, higher PSS concentration resulted in higher number of MyHC positive cells while culture medium containing lower PSS concentration promoted multi-nucleated myotube formation. (Scale bar: 100μm)
