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. 2014 Aug 18;9(8):e105354. doi: 10.1371/journal.pone.0105354

Figure 1. Sustained insulin treatment diminished the 11beta-HSD2 expression and activity in HT-29 cells.

Figure 1

(A) 11beta-HSD2 activity was measured by 3H-cortisol/cortisone conversion assay in colonic cell lines 24 h after incubation with insulin (10−11–10−7 M). The activity measured for HCT116 in absence of insulin was set as 100%. (B) Dose-response effect of insulin (10−9–10−5 M) on HSD11B2 mRNA (gray bars) and activity (curve) in HT-29 cells treated for 24 h. (C) Time-dependent effect of insulin (10−7 M) on HSD11B2 mRNA (gray bars) and activity (curve) in HT-29 cells. (D) Time-dependent effect of insulin (10−7 M) on 11beta-HSD2 protein level.