Skip to main content
. 2014 Aug 18;9(8):e105354. doi: 10.1371/journal.pone.0105354

Table 2. Probes used for the EMSA experiments with SP1.

Matrix SP-1 G(A/T)G(A)G G C(A/T)G G G(A/C)
cons SP-1 5′-attcgatcGGGGCGGGgcgagc-3′ (100%)
mut SP-1 5′-attcgatcGG TT CGGGgcgagc-3′ (80%)

The weight matrix for the consensus SP1 binding motif is given on top. “cons SP1” and “mut SP1” designate 22-mer oligonucleotides based respectively on the consensus and mutated binding site for SP1. Mismatched nucleotides with matrix are underlined. In bold are the nucleotides identical to the consensus sequence and the percentage of match with the consensus sequence is indicated.