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. 2014 Aug 18;9(8):e105011. doi: 10.1371/journal.pone.0105011

Figure 5. In vivo expression of vaccine-candidate genes.

Figure 5

Naïve adult female BALB/c mice (n = 4) were infected intranasally with B. pertussis strain B1917 or B1920. After 7 days, bacteria were collected through broncho-alveolar lavage and used for in vivo transcriptional analysis using antigen-specific primers as described in the text. The transcription data is expressed as 40-ΔCt value, which is a measure of expression relative to the recA household gene (ΔCt  =  Ct target – Ct recA). The number 40 represents the number of PCR cycles. A 40-ΔCt value of 40 indicates that the gene is expressed at equal levels as recA, while higher values correspond to higher expression.