Skip to main content
. 2014 Aug 18;9(8):e104890. doi: 10.1371/journal.pone.0104890

Figure 1. Effects of sildenafil treatment on histological changes of the lung.

Figure 1

Formalin fixed lungs of untreated (Sham), sildenagfil (Sham+SLD), MCT (PH) and MCT plus sildenafil treated (PH+SLD) rats (n = 3–4) were subjected to histopathological analysis. Representative HE stained sections (A), macrophage infiltration (B), the mean wall thickness of alveolar sac (C), representative high power HE images of a blood vessel (D) and representative CD-34 immunohistochemistry images (E) indicating vascularization and vascular remodeling are presented. Macrophages and blood vessels are indicated by filled and empty arrows, respectively. Bar values are mean±S.E.M. *** significantly different from Sham group (p≤0.05), +++ significantly different from PH group (p≤0.05) by ANOVA, post hoc Bonferroni test.