Table 1. Characteristics of DNA duplex thermal stabilities.
Duplex number (X/Y)a | Tmelt, °C (±1)b | h260, % (±1)c | CD, 10−6 Md | ΔT,°C (±1)e |
I (G/C) | 79 | 24 | 0.84 | 16 |
II (G/T) | 74 | 23 | 0.98 | 18 |
III (G/Tg) | 63 | 21 | 0.70 | 20 |
IV (A/Tg) | 67 | 21 | 0.62 | 19 |
Variable nucleotide pair.
Melting temperature of duplex.
Hyperchromic effect.
Concentration per duplex.
Cooperativity of phase transition.
The averaged data of three experiments are presented. The error of the mean indicates a SD.