Figure 6. Electrophoretic separation of PCR products from 1Bx promoters with or without the 43 bp insertion, and SDS-PAGE profiles of HMW-GS of wheat cultivars with the insertion.

(A) PCR assays for 1Bx promoters on a 2% agarose gel. M: 100 bp DNA Ladder. Underlined accessions possess the 43 bp insertion. (B) SDS-PAGE assay of HMW-GS from different accessions containing the 43 bp insertion. Underlined accessions possess the 43 bp insertion. Down black arrows indicate 1Bx7 with 43 bp insertion in accessions from China and Europe. Chinese Spring, Yanzhan 1 and Samo were used as controls. The purple curve represents the relative amounts from different 1Bx subunits.