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. 2014 Aug 18;9(8):e105185. doi: 10.1371/journal.pone.0105185

Figure 3. The hARD1 NLS mutant suppresses cell proliferation.

Figure 3

A. Constitutive expression of the NLS mutant of hARD1 leads to morphological alterations. HeLa cells transfected with hARD1 WT and ΔN were cultured in DMEM containing G418 to select for stable clones. After establishment, cell images were observed by phase-contrast microscopy. Arrows indicate cells with altered morphology (black, enlarged; red, rounded). Scale bar, 50 µm. B and C. hARD1ΔN-expressing cells showed a marked decrease in cell growth. B, cell growth of hARD1 WT and ΔN stable cell lines was monitored over time by MTS assay. The results are normalized to 0 day of each group and presented as mean ± S.D. (n = 5). C, HeLa cells stably expressing hARD1 WT and ΔN were subjected to anchorage-dependent (left) and -independent (right) colony formation assays. Number of colonies were presented as mean ± S.D. with representative well pictures. N = 3, ***P<0.005.