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. 2014 Aug 18;9(8):e105185. doi: 10.1371/journal.pone.0105185

Figure 4. The hARD1 NLS mutant causes alterations in cell cycle.

Figure 4

A. Deletion of NLS from hARD1 leads to moderate G2/M arrest in HeLa cells. Cell cycle profiles of hARD1 WT- and ΔN-expressing cells were determined by flow cytometry. Upper, representative cell cycle profiles; lower, the percentage of cells in each phase is presented as mean ± S.D. (n = 3). *p<0.05, **P<0.01 B. Deletion of NLS from hARD1 increased the protein levels involved in the G2/M phase and decreased those involved in the G0/G1 phase. The levels of Cyclin B1/E, Aurora kinase A/B (AURKA/B), and p53 were immunoblotted in the lysates extracted from hARD1 WT- and ΔN-expressing HeLa cells.