. 2014 Aug 18;9(8):e105088. doi: 10.1371/journal.pone.0105088

Table 2. Primers, T_m, extension time and cycles in RT-PCR program.

Gene	Accession No.	Primer	T_m (°C)	No. of cycles	Extension time (s)	Length of amplified DNA (bp)
GhCesA1	U58283	Forward: 5’- TGGGTTGAATGTTAATGGT-3’	58	30	60	632
		Reverse: 5’- CAGGATACCACTTAGGGAACT-3’
GhCesA2	U58284	Forward: 5'- CTGGCTTTGGTTCACTTGC-3'	58	30	60	529
		Reverse: 5'- CCGCCATTATCGTTGCTTA-3'
Gh18srR	U42827	Forward: 5’-CTGAGAAACGGCTACCACAT-3’	53	25	30	500
		Reverse: 5’-CTATGAAATACGAATGCCCC-3’

Table 2. Primers, Tm, extension time and cycles in RT-PCR program.