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. 2014 May 25;16(9):1229–1243. doi: 10.1093/neuonc/nou046

Fig. 6.

Fig. 6.

JAK2/STAT3 were key players in EGFRvIII-induced network and cell invasion in glioblastoma cells. (A) siRNA treatment on STAT3 in U87MG-EGFRvIII cells resulted in significant inhibition on total- and phosphorylated-JAK2 and EGFRvIII by Western blotting and (B) inhibition on cell invasion by transwell invasion assays. The data are shown as the mean ± standard error of the mean of at least 3 independent experiments. ** P < .01 compared with the scramble control. (C) Schematic illustration of a potential activation loop of EGFR, JAK2, and STAT3 in promoting MMP expression and cell invasion. (D) The treatment with siRNA on JAK2 and STAT3 but not AG490 (100 μM for 24 h) reduced the expression of MMP2 and MMP9 according to real-time PCR; and (E) treatment with siRNA on JAK2 and STAT3, and AG490 reduced the same expression by Western blotting in U87MG-EGFRvIII cells. Data are shown as the mean ± standard error of the mean of at least 3 independent experiments. ** P < .01, compared with the control.