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. 2014 Aug 18;9(8):e105444. doi: 10.1371/journal.pone.0105444

Figure 4. Developmental profile of RNase X25 transcript accumulation.

Figure 4

RNA was isolated from embryos at 0–2 h, 2–6 h, and 0–16 h after egg deposition and from animals at 3rd instar larval (L3), white prepupal (WPP), pupal (P), and adult male (M) or female (F) stages of development. Ovarian tissue (O) was prepared from 3–5 day old females. qPCR quantification of the relative level of RNase X25 mRNAs in these samples was carried out using the ribosomal protein L3 (RpL3) transcript as internal standard control for normalization. RNase X25 expression was detected at all stages analyzed. Data are representative of 3 independent experiments and are means and S.E. of triplicates.