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Conformational analysis of ConRl-A using RP-HPLC. (a) Effect of temperature on chromatography of the peptide at indicated temperature. The temperature of the C18 analytical column was maintained either by incubating in ice (or) water bath of the desired temperature. (b) Elution profiles obtained by reinjection of the fractions corresponding to Peak-1 and Peak-2 of the peptide at 4°C. Both major and minor conformers are represented from each peak.
