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. 2014 Aug 13;10:51. doi: 10.1186/1744-8069-10-51

Figure 5.

Figure 5

Methylation statuses of MOR gene distal promoter (DP) in DRG and the expression of MOR change after 5-aza-dC treatment. (A) The DP region of the MOR gene contains 11 putative methyl CpG sites from -1721 to -784. dpTIS indicates a TIS of the DP. (B, D) qRT-PCR show that MOR mRNA was increased in DRG after treatment with 5-aza-dC (5 μg, daily for 3 consecutive days, starting 30 minutes before surgery) (B), while no MOR mRNA increase was observed in the spinal cord (D). The ratio of MOR/GAPDH in Sham-Vehicle mice was set at 1 for quantifications. *p <0.05, **p <0.05 compared with Sham-Vehicle mice; ##p <0.01 compared with CCI-Vehicle mice, n=4 in each group. (C, E) Western blot shows that MOR protein was increased in DRG (C) and spinal cord (E) after treatment with 5-aza-dC (5 μg, daily for 3 consecutive days, starting 30 minutes before surgery). The ratio of MOR/GAPDH in Sham-Vehicle mice was set at 1 for quantifications. *p <0.05, **p <0.01 compared with Sham-Vehicle mice; ##p <0.01 compared with CCI-Vehicle mice, n=4 in each group.