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. 2014 Jun 14;307(4):H563–H573. doi: 10.1152/ajpheart.00250.2014

Fig. 3.

Fig. 3.

Neonatal chemical sympathectomy specifically suppresses MA maturation. Newborn rats were treated with vehicle or 6-hydroxydopamine (6-OHDA) to achieve chemical sympathectomy as described in materials and methods. MA (A–C) and aorta (D) were assayed at PND35. A: representative gels of Mypt1 E24 splice variants (plotted as %Mypt1 E24-). mRNA levels from PND35 vehicle vs. PND35 6-OHDA (B and C) MA and aorta (D) normalized to untreated PND12 arteries. E: fold change in protein levels in PND35 6-OHDA (n = 3) MA vs. PND35 vehicle control (n = 3) normalized to cyclophilin A, which was invariant. LZ-/LZ+ is the ratio of the signal obtained with the Mypt1 LZ- rabbit polyclonal antibody divided by the signal obtained on different blots with the Mypt1 LZ+ rabbit polyclonal antibody. All data are expressed as means ± SE. *P < 0.05 PND12 vs. PND35 vehicle, †P < 0.05 PND35 6-OHDA vs. PND35 vehicle. n = 5–6/group.