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. 2014 Jul 2;307(4):F435–F444. doi: 10.1152/ajprenal.00271.2014

Table 1.

Primers used in mRNA analysis

Gene Primer Sequence Accession No.
HPRT
    Sense 5′-GCTTACCTCACTGCTTTCCG-3′ NM_013556
    Antisense 5′-ATCATCGCTAATCACGACGC-3′
HK1
    Sense 5′-GGGACTATGACGCTAACATT-3′ NM_001146100
    Antisense 5′-CCAGTGCCAATGATCAGG-3′
HK2
    Sense 5′-GGTACAGAGAAAGGAGACTTC-3′ NM_013820
    Antisense 5′-TCTTGTTATGCATCTCTACGC-3′
HK3
    Sense 5′-CACTTAACCAATCTCGGAGT-3′ NM_001033245
    Antisense 5′-AGGCTATCACTTTCGATCTC-3′
GLUT1 (Slc2a1)
    Sense 5′-AGATGAAAGAAGAGGGTCGG-3′ NM_011400
    Antisense 5′-AGAACACAGCATTGATACCC-3′
PFKL
    Sense 5′-GATGTCTACCGTAAAGGGCG-3′ NM_008826
    Antisense 5′-ACTACCTTCTTCCGCAGGC-3′
PFKM
    Sense 5′-CAATCTGCAAGAAAGCAGCG-3′ NM_001163487
    Antisense 5′-GCAGCATTCATACCTTGGGC-3′
PGK1
    Sense 5′-CTGTGGTACTGAGAGCAGCAAGA-3′ NM_008828
    Antisense 5′-CAGGACCATTCCAAACAATCTG-3′
PKM
    Sense 5′-GGTGTTTGCATCTTTCATCCG-3′ NM_001253883
    Antisense 5′-AGATCTCATCAAACCTGCGG-3′
LDHA
    Sense 5′-CTTAATGAAGGACTTGGCGG-3′ NM_010699
    Antisense 5′-TGGTGTTTTAAGGAAGAGGC-3′
PDK1
    Sense 5′-ATCTCATCGAAAGCACATTGGA-3′ NM_172665
    Antisense 5′-CCGCCTAGCGTTCTCATAGC-3′