Fig. 1.

Effect of HDAC4 inhibition on HDAC4 proteins in cultured c-kit⁺ cardiac stem cells (CSCs). A: c-kit⁺ CSCs were transiently transfected with HDAC4 siRNA 1 (duplexes), HDAC4 siRNA2 and HDAC4 siRNA3 (500 nmol/l), or control siRNA (500 nmol/l) for 5 days to prepare RNA for real-time quantitative PCR to evaluate mRNA of HDAC4. B: protein lysates were prepared and HDAC4 proteins were measured. Densitometric analysis showing HDAC4 proteins in HDAC4 siRNA-transfected c-kit⁺ CSCs (n = 3 per group). Values represent means ± SE. *P < 0.05 vs. control siRNA. C: effect of HDAC4 overexpression on HDAC4 proteins in cultured c-kit⁺ CSCs. c-kit⁺ CSCs were infected with adenoviral GFP or adenoviral HDAC4 for 5 days to prepare protein lysates, and following immunoblotting assay, HDAC4 proteins were measured. D: densitometric analysis showing HDAC4 protein expression in adenoviral GFP or adenoviral HDAC4-infected c-kit⁺ CSCs (n = 3 per group).Values represent means ± SE. *P < 0.05 vs. adenoviral GFP.