FIG. 4.

Redifferentiation of P2 in SF3D. (A) Quantitative PCR analysis showed that cartilage-differentiation-associated genes in P2 cells were upregulated early. Col1a1 was downregulated by 48 h of culture when compared with freshly harvested P2-ML cells. Data are shown as mean with 95% CI (lower and upper limits are within brackets). n=3 experiments. (B) Representative immunoblots and densitometry of extracts from redifferentiating P2 cells in SF3D over the first 2 days of culture showed upregulation of insulin/IGF-pathway-related signaling molecules. (C) Glucose assay demonstrated higher uptake as indicated by increased intracellular glucose (2D-G ³H) by P2 cells cultured under LG serum-free conditions. HG-SF, serum-free high glucose content medium containing ITS; LG-SF, low glucose content media containing ITS; HG-20%, high glucose content medium with 20% fetal bovine serum. Each dot represents the mean of an independent experiment; horizontal lines represent mean and 95% CI. n=5 experiments. *p≤0.01, **p≤0.05.