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. 2014 Aug 19;4:6116. doi: 10.1038/srep06116

Figure 4. Ovary-secreted SDF-1 promotes the migration of OTICs.

Figure 4

(A). Quantitive RT-PCR of chemokines, cytokines, and growth factors in mouse ovaries. *P<0.05 vs the control ovaries (n = 3). (B). IHC staining of SDF-1 in mouse ovary. (Brown, SDF-1. Blue, nuclei). (C). Images of mCherry-OTICs that migrated to the lower chamber in migration assay. (D). Quantification of migrated mCherry-OTICs. *P<0.05 vs control group vs ★P<0.05 vs Lut-KGN CM group. (E). Western blots of p-ERK and total ERK in OTICs. GAPDH was used as loading control. The full-length blots are included in supplementary information. (Lut-KGN CM, luteinized KGN cell conditional medium. AMD3100, an SDF-1/CXCR4 inhibitor.)