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. 2014 Jul 8;25(8):730–739. doi: 10.1089/hum.2014.006

FIG. 3.

FIG. 3.

Expression of the ErbB2 TCR on retrovirally transduced SupT1 cells and CD8+ T cells. (A) Screening of TCR α/β pairs by retroviral transduction of SupT1 cells. Retroviruses encoding eight different TCR combinations were screened for ErbB2369–377 specificity by transduction of SupT1 cells. HLA-A2/ErbB2369–377 tetramer staining of the genetically modified SupT1 cells was performed 5 days after transduction and analyzed by flow cytometry. Two representative SupT1 populations are shown, each bearing different TCRs whose α and β chains were isolated from the ErbB2-specific polyclonal CD8+ T cells. Untransduced (NV) and MART1 SupT1 cells served as negative controls for HLA-A2/ErbB2369–377 tetramer binding. (B) HLA-A2/ErbB2369–377 tetramer staining of primary TCR-transduced CD8+ T cells. CD8+ T cells transduced with either the ErbB2 TCR7 or the MART1 TCR and untransduced CD8+ T cells (NV) were stained with the indicated HLA-A2/peptide tetramers. Numbers represent the percentage of tetramer+ cells. TCR, T-cell receptor.