Table 1.
TCR α and β chain sequencing resultsa | TCR α/β retroviral constructsb | ||||
---|---|---|---|---|---|
TRAV | Number of clones | TRBV | Number of clones | Construct number | TCR construct |
AV1-1 | 1 | BV2(22s1) | 1 | 1 | AV12-1 BV3-1(CB1) |
AV1-2 | 1 | BV3-1(9S1) | 2,3 | 2 | AV3 BV3-1(CB-1) |
AV2 | 1 | BV4-1(7S1) | 1 | 3 | AV12-2a BV3-1(CB-1) |
AV3 | 2 | BV4-3(7S2) | 1 | 4 | AV12-2b BV3-1(CB-1) |
AV10 | 1 | BV5-1(5S1) | 1 | 5 | AV12-2a BV3-1(CB-2) |
AV12-1 | 2,1 | BV5-4(5S6) | 1 | 6 | AV12-2b BV3-1(CB-2) |
AV12-2 | 1,1,1,1 | BV5-6(5S2) | 1 | 7 | AV3 BV3-1(CB-2) |
AV17 | 1 | BV20-1(2S1) | 1,1,1 | 8 | AV12-1 BV3-1(CB-2) |
AV21 | 1,1 | ||||
AV38-1 | 1 | ||||
AV38-2 | 1,1,1,1,1 |
TCR V α/β usage of HLA-A2/ErbB2 multimer+ CD69+ CD8+ T-cells. Twenty-three TCR α chain clones and 14 TCR β chain clones were isolated from ErbB2-specific CD8+ T-cells. The TRAV and TRBV repertoire was determined by sequencing. The number of repeats for each clone is shown on the right side of the table.
Eight different retroviral backbones encoding eight different TCR α/β combinations were constructed for the propagation of retroviral particles. TCR α and β chains that were presented more than once in the TCR repertoire were subcloned into the MSGV-1 retroviral backbones.
TCR, T-cell receptor; TRAV, T cell receptor α chain variable; TRBV, T cell receptor β chain variable.