Table 1.
T Cell Receptor α and β DNA Constructs
| TCR α and β chain sequencing resultsa | TCR α/β retroviral constructsb | ||||
|---|---|---|---|---|---|
| TRAV | Number of clones | TRBV | Number of clones | Construct number | TCR construct |
| AV1-1 | 1 | BV2(22s1) | 1 | 1 | AV12-1 BV3-1(CB1) |
| AV1-2 | 1 | BV3-1(9S1) | 2,3 | 2 | AV3 BV3-1(CB-1) |
| AV2 | 1 | BV4-1(7S1) | 1 | 3 | AV12-2a BV3-1(CB-1) |
| AV3 | 2 | BV4-3(7S2) | 1 | 4 | AV12-2b BV3-1(CB-1) |
| AV10 | 1 | BV5-1(5S1) | 1 | 5 | AV12-2a BV3-1(CB-2) |
| AV12-1 | 2,1 | BV5-4(5S6) | 1 | 6 | AV12-2b BV3-1(CB-2) |
| AV12-2 | 1,1,1,1 | BV5-6(5S2) | 1 | 7 | AV3 BV3-1(CB-2) |
| AV17 | 1 | BV20-1(2S1) | 1,1,1 | 8 | AV12-1 BV3-1(CB-2) |
| AV21 | 1,1 | ||||
| AV38-1 | 1 | ||||
| AV38-2 | 1,1,1,1,1 | ||||
a
TCR V α/β usage of HLA-A2/ErbB2 multimer+ CD69+ CD8+ T-cells. Twenty-three TCR α chain clones and 14 TCR β chain clones were isolated from ErbB2-specific CD8+ T-cells. The TRAV and TRBV repertoire was determined by sequencing. The number of repeats for each clone is shown on the right side of the table.
b
Eight different retroviral backbones encoding eight different TCR α/β combinations were constructed for the propagation of retroviral particles. TCR α and β chains that were presented more than once in the TCR repertoire were subcloned into the MSGV-1 retroviral backbones.
TCR, T-cell receptor; TRAV, T cell receptor α chain variable; TRBV, T cell receptor β chain variable.