FIG. 3.

Effect of adenosine on metabolic activity of a single C2C12 cell assessed through the MTS metabolic activity and dsDNA content (n=4). When treated with 5 mM adenosine under 0.1% hypoxic conditions, the metabolic activity was shifted to the right of the control group, resulting in its downregulated states approximately for the first 5 days. When these cells were transferred to normoxic conditions, the metabolic activity was restored equivalent to a normal level measured in normoxic cells. A normal level is shown only up to day 3 because the C2C12 cells became fully confluent at this time. MTS, 3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium. Color images available online at www.liebertpub.com/tea