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. 2014 Apr 1;20(15-16):2115–2126. doi: 10.1089/ten.tea.2013.0455

FIG. 2.

FIG. 2.

Morphology and orientation of human umbilical artery smooth muscle cells on engineered substrata. (A) Representative images demonstrating the morphology and orientation of the cells cultured and differentiated using smooth muscle differentiation supplement (SMDS)-supplemented vascular smooth muscle cell (vSMC) culture medium on different substrata, unpatterned or ANFS. Scale bar: 20 μm. (B) Depictions of cell orientation on the unpatterned and nanopatterned surfaces, the cells are clearly more directionally polarized on ANFS. n=100 (cells) for each condition. Statistically significant difference between nanopatterned and unpatterned substrata (p<0.05) for the same substratum stiffness is indicated with an asterisk. For all other substrata-type combinations, the identical signage indicates statistically significant difference for that pair. (C) Elliptical form factor (EFF) determinations indicated that vSMCs on unpatterned substrata were shorter and wider than the vSMCs cultured on ANFS; shape factor determinations indicated that the vSMCs on the stiffer ANFS were less circular than on the softer ANFS (p<0.05).