Table 1. Full Factorial Design Examined the Effect of Three Independent Variables on Kd of Equilibrium of Various Ligand–Protein Systemsa.
factor |
Kd (μM) ligand–protein |
|||||
---|---|---|---|---|---|---|
run | temp (°C) | NaCl (mM) | glycerol (%v/v) | SPD304/TNF-α | PABA/trypsin | RRLIF/cyclinA2 |
1 | 20 | 100 | 5 | 6.14 ± 0.32 | 4.83 ± 0.29 | 21.50 ± 1.52 |
2 | 20 | 100 | 50 | 22.54 ± 1.17 | 25.21 ± 1.55 | 32.24 ± 2.18 |
3 | 37 | 100 | 5 | 6.46 ± 0.38 | 6.41 ± 0.44 | N.D. |
4 | 37 | 100 | 50 | 19.18 ± 1.02 | 22.53 ± 1.37 | N.D. |
5 | 20 | 500 | 5 | 6.38 ± 0.43 | 6.23 ± 0.52 | 9.06 ± 0.81 |
6 | 20 | 500 | 50 | 24.93 ± 1.95 | 27.52 ± 1.38 | 33.00 ± 2.21 |
7 | 37 | 500 | 5 | 7.17 ± 0.64 | 7.62 ± 0.57 | N.D. |
8 | 37 | 500 | 50 | 21.87 ± 1.72 | 23.62 ± 0.31 | N.D. |
The mean values of three independent measurements are presented. N.D.: not determined.