Table 1. Full Factorial Design Examined the Effect of Three Independent Variables on K_d of Equilibrium of Various Ligand–Protein Systems^a.

factor				Kd (μM) ligand–protein
run	temp (°C)	NaCl (mM)	glycerol (%v/v)	SPD304/TNF-α	PABA/trypsin	RRLIF/cyclinA2
1	20	100	5	6.14 ± 0.32	4.83 ± 0.29	21.50 ± 1.52
2	20	100	50	22.54 ± 1.17	25.21 ± 1.55	32.24 ± 2.18
3	37	100	5	6.46 ± 0.38	6.41 ± 0.44	N.D.
4	37	100	50	19.18 ± 1.02	22.53 ± 1.37	N.D.
5	20	500	5	6.38 ± 0.43	6.23 ± 0.52	9.06 ± 0.81
6	20	500	50	24.93 ± 1.95	27.52 ± 1.38	33.00 ± 2.21
7	37	500	5	7.17 ± 0.64	7.62 ± 0.57	N.D.
8	37	500	50	21.87 ± 1.72	23.62 ± 0.31	N.D.

^aThe mean values of three independent measurements are presented. N.D.: not determined.