. 2014 Jul 9;5(8):931–936. doi: 10.1021/ml500204e

Table 2. Dissociation Constant Values for Three Different Complexes Calculated by Fluorescence Spectroscopy at Different Salt Concentrations^a.

	K_d (μM) ligand–protein
NaCl (mM)	SPD304/TNF-α	PABA/tryspin	RRLIF/cyclinA₂
50	5.42 ± 0.35	4.32 ± 0.31	N.D.
100	5.53 ± 0.51	4.12 ± 0.42	24.36 ± 1.25
250	5.71 ± 0.32	6.64 ± 0.35	17.45 ± 0.98
500	5.92 ± 0.42	8.58 ± 0.49	8.47 ± 0.62

Experiments performed in 10 mM citrate phosphate (pH 6.5) containing 5% DMSO, 50 mM Tris-HCl pH 8.0 containing 0.01% monothioglycerol, and 20 mM phosphate buffer (pH 7.4) containing 0.1% PEG6000, respectively, at 25 °C. The mean values of three independent measurements are presented. N.D.: not determined.

Table 2. Dissociation Constant Values for Three Different Complexes Calculated by Fluorescence Spectroscopy at Different Salt Concentrationsa.

Table 2. Dissociation Constant Values for Three Different Complexes Calculated by Fluorescence Spectroscopy at Different Salt Concentrations^a.