(A) Relative Vegfa mRNA expression in control (C) and mutant (M) MMTV-PyMT tumors at H/A, EC and LC stages. Inset: Immunoblotting for VEGFA and actin control and mutant MMTV-PyMT tumor lysates. (B) Relative sFlt-1 mRNA level in control and mutant MMTV-PyMT tumors at H/A, EC and LC stages. (C, D) Relative expression of Axin2, c-Myc, and CyclinD1 in control and mutant whole tumor (C) and CD31+, CD105+ blood vascular endothelial cells (D). Sample number is shown at the base of each histogram bar. Error bars are SEM.