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. Author manuscript; available in PMC: 2015 May 1.
Published in final edited form as: Crit Care Med. 2014 May;42(5):e355–e363. doi: 10.1097/CCM.0000000000000229

Figure 6. Inhibition of TRPM8 with “compound 5” potentiates and prolongs the hypothermic effect of TRPV1 activation by dihydrocapsaicin.

Figure 6

A) Mice were pretreated with TRPM8 inhibitor “compound 5”, 30 mg/kg i.p. (C5) or vehicle (Veh) 60 minutes prior to dihydrocapsaicin (DHC) injection. At time 0, mice were injected with dihydrocapsaicin 0.6, 1.25, and 2.5 mg/kg i.p. (arrow). Core temperature was measured for 5.5 hours (330 minutes) after dihydrocapsaicin injection. N=8 mice in each group. B) The minimum core temperature reached within the measurement period in each of the groups from experiment shown in A. * P<0.05 for vehicle versus ”compound 5” for the same dose of dihydrocapsaicin, Mann-Whitney rank sum test (for 1.25 mg/kg dihydrocapsaicin) or un-paired t-test (for 2.5 mg/kg dihydrocapsaicin). C) Time from the dihydrocapsaicin injection until core temperature recovered to 34 °C. Time was measured to the nearest 10 minutes. The graph shows individual values for each subject (symbols) and median values for each group (grey horizontal bar). Some mice did not recover the temperature of 34 °C within the measurement period (330 minutes) and were assigned the maximum possible value for the recovery time (330 minutes). A single mouse did not reach the hypothermic temperature of 34 °C and was assigned the minimum possible value for the recovery time (0 minutes). * P<0.01 for vehicle versus “compound 5” for the same dose of DHC, Mann-Whitney rank sum test.