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Significant difference in the number and size of neurons/neuronal nuclei (NeuN+) is evident if we compare (a) 2-month-old wt rats and (b) 18-month-old tgHD rats; (b) concomitant reactive astrogliosis is already developed in 18-month-old tgHD rats; it is also apparent that the degeneration of neurons in tgHD rats is typically selective (alike in human HD brain). Anti-NeuN (red) + anti-GFAP (green) + DAPI (blue). Bar 50 μm.
