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. 2014 Jun 12;31(9):2376–2386. doi: 10.1093/molbev/msu189

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© The Author 2014. Published by Oxford University Press on behalf of the Society for Molecular Biology and Evolution.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.

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Fig. 3. — Specific addition of poly(U) tails to transcripts of functional gene paralogs in the Karlodinium veneficum plastid. This gel photo shows the result of a series of RT-PCRs to identify whether transcripts of functional and pseudogenic copies of the rbcS and atpF genes in the Karl. veneficum plastid receive poly(U) tails. Lanes 1 and 2: oligo-d(A) RT-PCR of rbcS-1 (pseudogene) and rbcS-2 (functional). Lanes 3 and 4: RT-PCR of rbcS-1 with a gene-specific internal cDNA synthesis primer under template positive (lane 3) and negative (lane 4) conditions. Lanes 5 and 6: oligo-d(A) RT-PCR of atpF-1 (highly divergent gene) and the atpF-2 nonfunctional sequence between rps16 and psbB. Lanes 7 and 8: RT-PCR of the atpF-2 region with a gene-specific cDNA synthesis primer under template positive (lane 7) and negative (lane 8) conditions.