Fig. 4.

Mutations found in strains evolved against a drug class under strong selection (outer red circle) and mild selection (inner black circle) are shown with filled red and black markers, respectively. SNPs are shown with filled circles and indels are shown with filled triangles. Mutated genes’ names are printed using standard annotations; however, mutations are printed as “unknown” if there are no annotated genes found in literature (supplementary table S3, Supplementary Material online). Pathway-specific mutations are printed in blue. (A) Mutations found in strains evolved against 50S ribosomal inhibitors. (B) Mutations found in strains evolved against aminoglycosides. The TrkH gene, which is mutated in five aminoglycoside-resistant strains, is shown with a magenta arrow.