Table 1.
Potential biomarkers being detected in WF samples of the soft tissue or femoral bone defect.
| Description | UniProt accession | Gene ID | Description | MW [kDa] | Calc. pI | Max. Mascot score | Number of unique peptides | % sequence coverage | References | Further related proteins |
|---|---|---|---|---|---|---|---|---|---|---|
| Complement Factors | Q6P6G4 | C1qb | Complement C1q subcomponent subunit B | 26.6 | 8.8 | 42.1 | 2 | 11 | Cazander et al., 2012 [40] | C1qb, C2, C3, C4, C4b, C8g, and C9 |
| G3V615 | C3 | Complement C3 | 186.2 | 6.5 | 2128.3 | 87 | 57 | Cazander et al., 2012 [40] | ||
|
| ||||||||||
| Serin proteases and inhibitors |
Q5RKH1 Q3T1J1 |
Prtn3 ELANE |
Protein Prtn3 elastase 2, neutrophil |
27.7 29.5 |
8.0 9.3 |
48.9 96.1 |
2 7 |
16 28 |
Eming et al., 2007 [41] Buchstein et al., 2009 [42] |
A1m, A2m, SERPINA4, SERPINC1, SERPINF2, Ambp, C3, C4, C4b, C8g, Itih1, Itih3, and Itih4 |
|
| ||||||||||
| Cytokine receptor antagonists | P20761 | Il1rn | Interleukin-1 receptor antagonist protein | 20.3 | 6.9 | 51.5 | 3 | 17 | Ishida et al., 2006 [43] | |
|
| ||||||||||
| Matrix metallopeptidases | P08011 | Mmp8 | Matrix metallopeptidase 8 | 53.2 | 6.6 | 42.7 | 5 | 16 | Gutiérrez-Fernández et al., 2007 [44] | Anpep, Cpb2, Hpx, and Lta4h |
| G3V7D0 | Mmp9 | Matrix metallopeptidase 9 | 78.5 | 6.3 | 65.6 | 4 | 8 | Reiss et al., 2010 [45] | ||
|
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| Oxidoreductases | D3ZYK8 | Mpo | Myeloperoxidase (mapped) | 51.9 | 9.9 | 798.4 | 9 | 66 | Moor et al., 2009 [7] | Gpx1, Gpx3, Gsr, HMOX1, Impdh2, Lbr, Ldha, Ldhb, Ldhc, Mdh1, Mdh2, Mpo, Mtco2, Ndufv2, Nos2, Pgd, Prdx1, Prdx2, Prdx6, Sod1, Sod2, Sod3, Tkt, and Txn |
|
| ||||||||||
| S100 proteins and annexins | Q6B345 | S100a8 | Protein S100-A8 | 10.2 | 6.1 | 426.6 | 7 | 87 | Thorey et al., 2001 [38] | S100a11, Anxa2, Anxa3, Anxa4, Anxa5, Anxa6, Anxa7, and Anxa11 |
| P50115 | S100a9 | Protein S100-A9 | 13.1 | 7.5 | 375.5 | 15 | 84 | Wyffels et al., 2010 [20] | ||
| G3V7W7 | Anxa1 | Annexin A1 | 38.8 | 7.3 | 1750.0 | 33 | 75 | Leoni et al., 2013 [46] | ||
Proteins which were reported as biomarker candidates and could be identified in at least 2 samples of the soft tissue or femoral bone defect are grouped according to their annotated protein classes. Additionally, further proteins being identified and belonging to the same protein classes are listed.