Table 2.
ssDNA binding properties of KpSSB, StSSB, PaSSB, KpSSBnStSSBc, and KpSSBnPaSSBc as analyzed by EMSA.
| Protein | DNA | [Protein]50 (nM) | Complex number |
|---|---|---|---|
| KpSSB | dT20 | ND | 0 |
| dT25 | 200 ± 20 | 1 | |
| dT35 | 220 ± 30 | 1 | |
| dT45 | 100 ± 10 | 1 | |
| dT50 | 110 ± 20 | 1 | |
| dT55 | 100 ± 20 | 2 | |
| dT60 | 100 ± 10 | 2 | |
|
| |||
| StSSB | dT15 | 650 ± 120 | 1 |
| dT20 | 450 ± 80 | 1 | |
| dT30 | 420 ± 60 | 1 | |
| dT40 | 420 ± 80 | 1 | |
| dT45 | 440 ± 60 | 2 | |
| dT50 | 440 ± 50 | 2 | |
|
| |||
| PaSSB | dT20 | ND | 0 |
| dT25 | 1700 ± 250 | 1 | |
| dT35 | 950 ± 180 | 1 | |
| dT45 | 780 ± 160 | 1 | |
| dT55 | 820 ± 90 | 1 | |
| dT60 | 810 ± 110 | 2 | |
| dT65 | 550 ± 70 | 2 | |
|
| |||
| KpSSBnStSSBc | dT15 | 260 ± 60 | 1 |
| dT20 | 110 ± 20 | 1 | |
| dT40 | 120 ± 20 | 1 | |
| dT45 | 160 ± 20 | 2 | |
|
| |||
| KpSSBnPaSSBc | dT20 | ND | 0 |
| dT25 | 390 ± 60 | 1 | |
| dT40 | 220 ± 30 | 1 | |
| dT55 | 230 ± 30 | 1 | |
| dT60 | 230 ± 30 | 2 | |
[Protein]50 was calculated from the titration curves of EMSA by determining the concentration of the protein (μM) needed to achieve the midpoint value for input ssDNA binding. For some oligonucleotides, input ssDNA binding was the sum of the intensities from the two separate ssDNA-protein complexes. Errors are standard deviations determined by three independent titration experiments.