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. 2014 Jan 22;7(5):1127–1138. doi: 10.1038/mi.2013.128

Figure 7.

Figure 7

Interferon-stimulated gene of 15 kDa (ISG15) does not alter human rhinovirus (HRV)-16-induced RIG-I protein expression, but ISG15 binding to RIG-I is increased following HRV infection. (a) HRV-16-induced RIG-I and MDA-5 protein levels in human bronchial epithelial (HBE) cell lysates (48 h after infection) were assessed by western blotting after lipid-mediated transfection (24 h) of 10 nM ISG15 short-interfering RNA (siRNA) duplexes A or B, compared with control, nontargeting siRNA. (b) HRV-16 infection induces ISG15 binding to RIG-I in BEAS-2B cells. A pTIS plasmid encoding FLAG-tagged RIG-I was transiently transfected using lipid-mediated techniques. Cells were infected with HRV-16 for 48 h and cells were lysed. (c) HRV-16-induced ISG15 and RIG-I protein levels (24 h after infection) in HBE cells were reduced after lipid-mediated transfection (24 h) of 10 nM RIG-I siRNA duplexes A or B compared with control, nontargeting siRNA. Blots are representative of three separate experiments. Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) was used as an internal control. M, medium control; HRV, HRV-16.