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. Author manuscript; available in PMC: 2015 Sep 1.
Published in final edited form as: J Immunol. 2014 Jul 21;193(5):2317–2329. doi: 10.4049/jimmunol.1303223

Figure 6. GMCSF-MOG targeted MOG35-55 for inhibitory MHCII-restricted antigen presentation by inflammatory DC.

Figure 6

C57BL/6 BM cells were cultured at 1 × 106 cells/ ml with 1 μM additions of GMCSF-MOGor a combination of GM-CSF and MOG35-55(GMCSF+MOG) for 4 days. BM cells were extensively washed and added to wells (x-axis plot of exponent) at designated densities (cells/ well) of 80,000 (104.9), 40,000 (104.6), 20,000 (104.3), 10,000 (104.0), 5,000 (103.7), 2,500 (103.4), 1,250 (103.1), or no DC in the presence (B, C) or absence (A, C) of an anti-I-Ab mAb (Y3P, 2% v/v) with (C) or without (A, B) 1 μM MOG35-55. MOG-specific 2D2 T cells were added at a density of 25,000/ well. Cultures were pulsed with [3H]thymidine on day 2 of a 3-day culture as a measure of cellular proliferation. Note that the y-axis scale for A-B differs from that for C. These data are representative of three independent experiments.