Table 2.
Effect of expression of mutated phospholipase A2 (mPLA2) in transgenic Aedes fluviatilis on Plasmodium gallinaceum oocyst formation
| Experiment | Mosquitoes | Prevalence† | Oocyst‡ | Inhibition§ |
|---|---|---|---|---|
| 1 | Control | 96% (43/45) | 51.9 (0–170) | – |
| 4T | 86.2%(25/29) | 26 (0–63) | 49.9%* | |
| 2 | Control | 96.4% (27/28) | 41.9 (0–148) | – |
| 4T | 75% (21/28) | 13.4 (0–136) | 68%* | |
| 3 | Control | 90% (29/32) | 83.6 (0–287) | – |
| 4T | 86% (19/22) | 40.1 (0–168) | 52%* | |
| 4 | Control | 87.5% (35/40) | 104.75 (0–240) | – |
| 4T | 71% (16/21) | 65.6 (0–259) | 37.3%* | |
| 5 | Control | 80.6% (25/31) | 26.4 (0–136) | – |
| 8T | 75.6% (31/41) | 19.4 (0–120) | 26.5%* | |
| 6 | Control | 74% (20/27) | 12.7 (0–50) | – |
| 8T | 59% (13/22) | 4 (0–44) | 68.5%* | |
| 7 | Control | 85.2% (41/49) | 48 (0–213) | – |
| 8T | 75% (23/30) | 34 (0–200) | 21.9%* | |
| 8 | Control | 83.3% (30/36) | 34.8 (0–160) | – |
| 9T | 71.4% (15/21) | 20.5 (0–64) | 41.2%* | |
| 9 | Control | 100% (39/39) | 49.8 (1–172) | – |
| 9T | 80.9% (17/21) | 41.2 (0–184) | 17.5%* | |
| 10 | Control | 78.3% (18/23) | 11.9 (0–71) | – |
| 9T | 65% (13/20) | 8.6 (0–40) | 27.7%* | |
| 11 | Control | 87.5% (21/24) | 7.5 (0–56) | – |
| 10T | 81.8% (9/11) | 3.8 (0–9) | 49.3%* | |
| 12 | Control | 93% (38/40) | 63.8 (0–212) | – |
| 10T | 50% (10/20) | 27.1 (0–124) | 57.5%* | |
| 13 | Control | 100% (32/32) | 120.4 (12–291) | – |
| 10T | 85.7% (19/21) | 84.4 (0–198) | 30%* |
†
Percentage of infected (actual numbers in parentheses).
‡
Arithmetic mean of oocyst number per mosquito gut. The range of observed values is indicated in parentheses.
§
100 – [(mean oocyst number in transgenic mosquitoes/mean oocyst number in control mosquitoes) × 100].
*
Reduction of oocyst number/gut was significant (P-value < 0.05) for all lines, as calculated by the Mann–Whitney U test.