Skip to main content
. Author manuscript; available in PMC: 2015 Feb 21.
Published in final edited form as: ACS Synth Biol. 2014 Jan 9;3(2):83–90. doi: 10.1021/sb400166j

Figure 4. Human HT1080 cells carrying the 21HAC2 and tet-O HAC2 vectors expressing the EGFP and DsRed genes.

Figure 4

(a) Scheme of transferring the EGFP-21HAC2 and tet-O-DsRed-HAC2 vectors and elimination of the tet-O-DsRed-HAC2 vector after expression of the tTS gene. (b) FISH analysis of the HT72 cells carrying EGFP-21HAC2 and tet-O-DsRed-HAC2 vectors. FISH analysis was performed using a biotin-labeled probe for the DsRed gene (green) and digoxigenin-labeled p11-4 (a hChr.21-derived α-satellite clone) (red). (c) Bright-field and fluorescence images of HT72 cells after elimination of the tet-O-DsRed-HAC2 vector. (d) FACS analysis was performed to detect the EGFP and DsRed genes expression 7 days after introduction of the tTS gene. (e) The retention rate of the EGFP-21HAC2 and tet-O-DsRed-HAC2 vectors was calculated by FISH analysis 7 days after introduction of the tTS gene. 200 interphase were counted for calculating the retention rate of HACs.