Figure 2.
Lipopolysaccharide (LPS) induction of vasoactive intestinal peptide receptor (VPAC)2 expression in patients' CD14+ cells and vasoactive intestinal peptide (VIP) modulation. Monocytes from different primary Sjögren's syndrome (pSS) patients or control subjects (control) were isolated and incubated for 24 h with 1 μg/ml LPS in the presence or absence of 100 nM VIP as described in Material and methods before they were analysed for VPAC2 expression by quantitative reverse transcription–polymerase chain reaction (qRT–PCR). Values represent the fold increase of VPAC2 expression in each patient or control monocyte sample incubated with LPS for 24 h with or without VIP, over VPAC2 expression in the same incubation period in the absence of stimuli. *P < 0·05, Mann–Whitney U-test.