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. Author manuscript; available in PMC: 2014 Aug 19.
Published in final edited form as: J Healthc Eng. 2012 Dec;3(4):503–534. doi: 10.1260/2040-2295.3.4.503

Figure 3.

Figure 3

The VeraTag assay detects binding of nearby epitopes. A) Two proteins that bind to each other are incubated with a fluorescently tagged antibody (labeled F) and a biotin tagged antibody (labeled B). B) The tagged antibodies bind the proteins, bringing them into proximity with each other. C) A streptavidin probe (labeled S) linked to a photosensitizer binds to the biotin linked antibody. D) Exposure to light in the presence of a photosensitizer excites oxygen into the singlet state, causing localized cleavage of the fluorescent tag from the fluorescently tagged antibody. E) The released fluorescent tag can be detected by capillary electrophoresis.