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. Author manuscript; available in PMC: 2014 Aug 19.
Published in final edited form as: Radiat Res. 2014 Jul 3;182(2):163–169. doi: 10.1667/RR13471.1

FIG. 1.

FIG. 1

Effect of local tumor irradiation alone or in combination with cyclophosphamide on growth of 4T1 breast tumors. Panel A: Survival of BALB/C mice given subcutaneous injections of 1 × 104 4T1 wild-type tumor cells and 4T1 luc+ tumor cells compared to survival of 4T1 WT or 4T1 luc+ tumor-bearing mice given 60 Gy (3 daily doses of 20 Gy each) local tumor irradiation (LTI) on days 14, 15 and 16. There were five mice in each group. Panel B: Bioluminescence imaging (BLI) of mice at serial time points after injection of 4T1 luc+ tumor cells. Note signals above diaphragm at days 21 and 28. Panel C: Representative tissue section (H&E, 1003 magnification) of lung showing tumor cell cluster (arrows) in untreated mouse. Panel D: BLI of mice given 4T1 luc+ tumor cells after 60 Gy (3 daily doses of 20 Gy each) local tumor irradiation on days 14, 15 and 16 or on days 21, 22 and 23. Blank areas indicate death of mice. Panel E: BLI of mice given 4T1 luc+ cells, 60 Gy (3 daily doses of 20 Gy each) local tumor irradiation and intraperitoneal injection of cyclophosphamide (500 mg/ kg) on day 23. There were 5–8 mice in each group in D and E.