Figure 5.

Immunoblots using sera from 2 representative 6-week-old puppies with low maternal antibodies before (a and c) and 14 days after (b and d) aerosol challenge with Bordetella bronchiseptica (Bb). Immunoblots using sera from representative adult Bb-low seropostitive laboratory dog before (e) and 28 days after vaccination (on days 0 and 14) with acellular Bb vaccine. Immunoblots using sera from representative adult Bb-low seropositive client-owned dog before (g) and 10 days after single vaccination (h) with acellular Bb vaccine. Lines indicate relative molecular weight of proteins in kD. Arrows indicate predicted location of pertactin (p68, thick arrow) and fimbrial hemagglutinin (p220, thin arrow).