Table 2.

Cocktail for PCR amplification

Constituents	SLCO1B1 388A>G	SLCO1B1 521T>C
Template	50 ng	50 ng
10X PCR buffer	1X	1X
MgCl2 (25 mM)	3 mM	5 mM
dNTP’s	0.2 mM	0.2 mM
Outer F primer (10 pmol/μl)	1 pmol	1 pmol
Specific F Primer (10 pmol/μl)	1 pmol	1 pmol
Outer R primer (10 pmol/μl)	1 pmol	1 pmol
Specific R primer (10 pmol/μl)	1 pmol	1 pmol
Taq DNA Polymerase (2 U/μl)	10 U	10 U
dH2O (nuclease free)	Upto 50 μl	Upto 50 μl
Total reaction mixture (μl)	50	50