Table 2.
Mutation libraries for high-throughput epitope mapping
| Class | Target protein | Library size (no. of clones) | Library type |
|---|---|---|---|
| Virus | DENV-3 prM/E | 1400 | 2 mutations/AA |
| Virus | DENV-4 prM/E | 660 | Ala scan |
| Virus | CHIKV E2/E1 | 920 | Ala scan |
| Virus | HCV E1/E2 | 553 | Ala scan |
| Virus | HIV gp160 | 679 | Ala scan |
| Virus | RSV F protein | 1029 | 2 mutations/AA |
| Virus | HBV sAg | 441 | 2 mutations/AA |
| GPCR | CXCR2 | 714 | Ala scan |
| GPCR | CXCR4 | 731 | 2 mutations/AA |
| GPCR | CCR5 | 734 | 2 mutations/AA |
| GPCR | TAS2R16 | 573 | 2 mutations/AA |
| 4TM | Claudin-1 | 413 | 2 mutations/AA |
| 4TM | Claudin-4 | 423 | 2 mutations/AA |
| Membrane | MCAM | 545 | Ala scan |
| Membrane | Her-2 | 625 | Ala scan |
DENV, dengue virus; CHIKV, chikungunya virus; GPCR, G protein-coupled receptor; HCV, hepatitis C virus; HBV, hepatitis B virus; HIV, human immunodeficiency virus; RSV, respiratory syncytial virus.
Comprehensive mutation libraries constructed at Integral Molecular cover nearly every residue of the indicated proteins, over 10 000 individual mutations in total. Initial libraries were derived using random mutagenesis and selection of two or three mutants per position, while subsequent libraries have been constructed using alanine scanning mutagenesis.