Table 1.

Interleukin-32γ (IL-32γ)-induced protein phosphorylations

Phosphoprotein	Kinase target	IL-32γ-induced relative fold change	P-value
Fyn	Y420	3·43	P < 0·01
Akt-1	T308	3·26	P < 0·01
MKP-1	S359	2·52	P < 0·03

Human macrophage-like THP-1 cells were stimulated with IL-32γ (20 ng/ml) for 15 min. A kinome screen using peptide arrays representing 300 phosphorylation targets of kinase activity was employed for quantifying kinase activity.⁴⁸ Table shows proteins that were phosphorylated ≥ 2·5 fold (P < 0·05) in the presence of the cytokine IL-32γ compared with unstimulated cells