Figure 4.

Dendritic cells (DC) differentiated in the presence of low concentrations of interferon-γ (IFN-γ^Low) possess a distinct molecular signature. (a) Principal component analysis (PCA) of the transcriptome of peripheral blood monocytes from four healthy donors differentiated into DC with either granulocyte–macrophage colony-stimulating factor (GM-CSF) and interleukin-4 (IL-4) or GM-CSF, IL-4 and IFN-γ^Low reveals the heterogeneity of donors (indicated by colour) and the global transcriptome changes (distance between the same colour dots). (b) Dendrogram of whole transciptome analysis displaying 461 genes differentially expressed with a P-value cut-off of 0·01 for moDCs differentiated with GM-CSF and IL-4 or GM-CSF, IL-4 and IFN-γ^Low. (c) Differential expression of transcriptional networks involving POU6F1, E2F2 and KLF5 following exposure to low levels of IFN-γ during monocyte differentiation into DC. Red = up-regulated genes; Green = down-regulated genes.