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. 2014 Jul 29;143(1):120–129. doi: 10.1111/imm.12294

Figure 1.

Figure 1

Effect of sialoadhesin-deficiency on follicular dendritic cell (FDC) status. Splenic marginal zone (a) and lymph node sub-capsular sinus macrophages (c) express high levels of sialoadhesin (green) in wild-type (WT) mice, whereas sialoadhesin is undetectable in the spleen (b) and lymph nodes (d) of sialoadhesin-deficient (Sn−/−) mice. Sections were also immunostained to detect B cells (B220+ cells, blue). Immunostaining with anti-CD35 (e, f, red) revealed that sialoadhesin-deficiency had no effect on FDC network size (g). Comparison of sialoadhesin (blue), complement component C4 (green) and PrPC-expression (red) in the spleens of WT (h) and Sn−/− (i) mice. In the spleens of Sn−/− mice, morphometric analysis revealed significant increases in the level of complement component C4 on the surfaces of FDC (j) and their expression of PrPC (k) when compared with WT mice. *P < 0·001; **P < 0·0001.