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. Author manuscript; available in PMC: 2014 Aug 19.
Published in final edited form as: Stress. 2009 May;12(3):250–258. doi: 10.1080/10253890802367265

Table I.

Example of prenatal stress regimen.

Gestational day
14 15 16 17 18 19 20
Female 1 AM Restraint Injection Restraint Novel cage Novel cage Restraint Injection
PM Novel cage Injection Injection Restraint Novel cage Novel cage Restraint
Female 2 AM Restraint Injection Restraint Novel cage Restraint Restraint Injection
PM Restraint Injection Restraint Novel cage Novel cage Restraint Injection
Female 3 AM Injection Restraint Restraint Injection Novel cage Novel cage Novel cage
PM Restraint Injection Restraint Novel cage Novel cage Restraint Injection

Each dam received a random stressor during the AM hours (0700–1200 h) and a second stressor during the PM hours (1300–1900 h). Selection of stressors was random and varied between dams. Gestational stressors consisted of a saline injection (0.9% NaCl, 0.1 ml, i.m.), handling and placement into a novel environment for 10 min, and placement into a plexiglas restraining chamber under bright light for 10 min.