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. Author manuscript; available in PMC: 2014 Aug 19.
Published in final edited form as: World J Surg. 2011 Aug;35(8):1715–1724. doi: 10.1007/s00268-010-0939-9

Fig. 4. SSTR5 L335 has no effect on the inhibition of glucose-stimulated insulin secretion by RPL-1980 in insulinoma β-TC6 cells.

Fig. 4

β-TC6 cells were transfected with empty vector, HA-SSTR5 P335 or HA-SSTR5 L335 using Lipofectamine 2000. Twenty-four (24) h after transfection, the cells were washed twice with Krebs-Ringer bicarbonate (KRB) buffer and incubated in KRB-BSA for 1 h. The cells were then added to 11.1 mM of glucose and 10-6 M of SSTR5 analogue PRL-1980 and incubated for 2 h. After incubation, the media were collected and centrifuged at 600 g for 5 min. The insulin concentrations in the media were measured by ELISA.