Table 1. Strains used for specificity testing.

M. absessus ATCC 19977	M. mageritense 981294	Arcanobacterium hemolyticum
M. aurum ATCC 23366*	M. malmoense ATCC 29571	Bordetella cepacia ATCC 25410
M. avium 961091*	M. malmoense 40445*	B. pertussis
M. avium ATCC 25291	M. marina ATCC 927	B. bronchiseptica ATCC 19395
M. brisbanense	M. marinum1717	Corynebacterium striatum
M. catarrhalis	M. nebraskense	Escherichia coli ATCC 25922
M. chelonae	M. neoaureum 40853	Gordonia bronchialis
M. chelonae 41426	M. nonchromogenicum 970454*	Haemophilus influenzae
M. chitae ATCC 19628*	M. nonchromogenicum ATCC 19530	Legionella pneumophilia ATCC 33152
M. diernhoferi ATCC 19340*	M. parafinicum 3098*	Neisseria meningitidis ATCC 13077
M. fortuitum ATCC 6841	M. peregrinum	Pseudomonas aeruginosa
M. gordonae ATCC 14470	M. peregrinum 10086*	Rhodococcus equi
M. gordonae10284	M. poriferae ATCC 35087	Staphylococcus aureus ATCC 29213
M. heidelbergense 981295*	M. scrofulaceum ATCC 19073*	Streptococcus agalactiae ATCC 27591
M. immunogen 981291*	M. shimodei 27962*	S. anginosus ATCC 33397
M. intracellulare 32665*	M. simiae ATCC 25273	S. pneumoniae ATCC49619
M. intracellulare ATCC 13950	M. smegmatis mc2155*	S. pyogenes ATCC 12370
M. intracellulare ATCC 35761	M. szulgai ATCC 35799	S. salivarus
M. kansasii	M. szulgai 4981*	Yersinia pseudotuberculosis ATCC 23207
M. kansasii ATCC 12478	M. ulcerans 5147
M. kansasii MYC 4296	M. xenopi
M. lentifalvum 960190*	M. xenopi 9741*
M. liflandi 40413*

Non-tuberculous mycobacterial (NTM) strains and other bacterial species used in the determination of the specificity of the IS6110 and IS1081 RPA assays. All tested negative by both RPA assays. Isolates marked * were supplied by the Mycobacteriology Unit, Institute of Tropical Medicine, Antwerp, Belgium. All other isolates were from the Washington State Public Health Laboratory strain collection.